pc3.1-CAGGS-ASAP3-mCyRFP3
(Plasmid
#193323)
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PurposeA red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 193323-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 193323-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 6103
- Total vector size (bp) 2007
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameASAP3-R3
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SpeciesG. gallus (chicken); jelly fish, Entacmaea quadricolor
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Insert Size (bp)2007
- Promoter caggs
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene NGS result contains ambiguous bases in the CAG promoter due to its GC-rich nature. Addgene's maps are automatically annotated by SnapGene. The mCyRFP3 fluorophore may be mis-annotated since not all variants are included on the common list of features. Please see the Annotated Depositor Map in the Supplemental Documents section for reference.
DNA (Catalog # 193323-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 193323-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pc3.1-CAGGS-ASAP3-mCyRFP3 was a gift from Michael Lin (Addgene plasmid # 193323 ; http://n2t.net/addgene:193323 ; RRID:Addgene_193323) -
For your References section:
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3. Kim BB, Wu H, Hao YA, Pan M, Chavarha M, Zhao Y, Westberg M, St-Pierre F, Wu JC, Lin MZ. Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.1038/s41598-022-07313-1. 10.1038/s41598-022-07313-1 PubMed 35256624
Map uploaded by the depositor.
