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Addgene

GFPxm163
(Plasmid #193480)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193480 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1(+)-N-6His
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFPxm163
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.07.08.499230 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFPxm163 was a gift from Sheel Dodani (Addgene plasmid # 193480 ; http://n2t.net/addgene:193480 ; RRID:Addgene_193480)

  • For your References section:

    Discovery of a monomeric green fluorescent protein sensor for chloride by structure-guided bioinformatics. Peng W, Maydew CC, Kam H, Lynd JK, Tutol JN, Phelps SM, Abeyrathna S, Meloni G, Dodani SC. Chem Sci. 2022 Oct 6;13(43):12659-12672. doi: 10.1039/d2sc03903f. eCollection 2022 Nov 9. 10.1039/d2sc03903f PubMed 36519056
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