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(Plasmid #80742)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 80742 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Total vector size (bp) 4800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    YFP with enhanced sensitivity to low chloride concentrations (YFPQS)
  • Species
  • Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
  • Promoter CMV
  • Tag / Fusion Protein
    • palmitoylation signal sequence of neuromodulin (N terminal on backbone)

Cloning Information

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mbYFPQS was a gift from Susan Amara & Susan Ingram (Addgene plasmid # 80742 ; ; RRID:Addgene_80742)
  • For your References section:

    A sensitive membrane-targeted biosensor for monitoring changes in intracellular chloride in neuronal processes. Watts SD, Suchland KL, Amara SG, Ingram SL. PLoS One. 2012;7(4):e35373. doi: 10.1371/journal.pone.0035373. Epub 2012 Apr 10. 10.1371/journal.pone.0035373 PubMed 22506078