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Purposemembrane-targeted biosensor for monitoring changes in intracellular chloride
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Depositing Labs
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Depositing OrganizationOregon Health & Science University
Located in United States of America -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 80742-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 80742-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEYFP-Mem
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Backbone manufacturerClontech
- Total vector size (bp) 4800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFPQS
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Alt nameYFP with enhanced sensitivity to low chloride concentrations (YFPQS)
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SpeciesSynthetic
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MutationH148Q (CAC to CAG) and V163S (GTG to AGC).
- Promoter CMV
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Tag
/ Fusion Protein
- palmitoylation signal sequence of neuromodulin (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 80742-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 80742-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mbYFPQS was a gift from Susan Amara & Susan Ingram (Addgene plasmid # 80742 ; http://n2t.net/addgene:80742 ; RRID:Addgene_80742) -
For your References section:
A sensitive membrane-targeted biosensor for monitoring changes in intracellular chloride in neuronal processes. Watts SD, Suchland KL, Amara SG, Ingram SL. PLoS One. 2012;7(4):e35373. doi: 10.1371/journal.pone.0035373. Epub 2012 Apr 10. 10.1371/journal.pone.0035373 PubMed 22506078