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(Plasmid #19363)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 19363 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Vernet et al 1987
  • Backbone size (bp) 6400
  • Modifications to backbone
    SfiIA (GGCCATTACGGCC) and SfiIB (GGCCGCCTCGGCC) sites added for unidirectional cloning.
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG300 was a gift from Holger Deising (Addgene plasmid # 19363 ; ; RRID:Addgene_19363)
  • For your References section:

    Sfp-type 4'-phosphopantetheinyl transferase is indispensable for fungal pathogenicity. Horbach R, Graf A, Weihmann F, Antelo L, Mathea S, Liermann JC, Opatz T, Thines E, Aguirre J, Deising HB. Plant Cell. 2009 Oct;21(10):3379-96. doi: 10.1105/tpc.108.064188. Epub 2009 Oct 30. 10.1105/tpc.108.064188 PubMed 19880801