pRC3EGFP
(Plasmid
#193790)
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PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepZ
- Backbone size w/o insert (bp) 1990
- Total vector size (bp) 2710
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesBacteria
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Insert Size (bp)720
- Promoter PR promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CAAGGGCGAGGAGCTGTT
- 3′ sequencing primer CCATGCCGAGAGTGATCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRC3EGFP was a gift from Sangram Bagh (Addgene plasmid # 193790 ; http://n2t.net/addgene:193790 ; RRID:Addgene_193790) -
For your References section:
A frame-shifted gene, which rescued its function by non-natural start codons and its application in constructing synthetic gene circuits. Sarkar K, Mukhopadhyay S, Bonnerjee D, Srivastava R, Bagh S. J Biol Eng. 2019 Mar 1;13:20. doi: 10.1186/s13036-019-0151-x. eCollection 2019. 10.1186/s13036-019-0151-x PubMed 30867677
Map uploaded by the depositor.
