pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)
(Plasmid
#194190)
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PurposeIncludes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)
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Depositing Labs
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Depositing OrganizationGoethe-Universitaet Frankfurt am Main
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 194190-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 194190-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSMANTIS promoter (1kb)
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Alt nameMANTIS promoter (1kb)
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Alt nameAK125871 promoter (1kb)
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SpeciesH. sapiens (human)
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Mutation5 mutated KLF binding sites (positions 449,548,732,899,884)
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Entrez GeneSMANTIS (a.k.a. ANXA4-AS, n342419)
- Promoter SMANTIS promoter (1kb), mutated
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 194190-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 194190-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All) was a gift from Ralf Brandes & Matthias Leisegang (Addgene plasmid # 194190 ; http://n2t.net/addgene:194190 ; RRID:Addgene_194190) -
For your References section:
Pleiotropic effects of laminar flow and statins depend on the Kruppel-like factor-induced lncRNA MANTIS. Leisegang MS, Bibli SI, Gunther S, Pfluger-Muller B, Oo JA, Hoper C, Seredinski S, Yekelchyk M, Schmitz-Rixen T, Schurmann C, Hu J, Looso M, Sigala F, Boon RA, Fleming I, Brandes RP. Eur Heart J. 2019 Aug 7;40(30):2523-2533. doi: 10.1093/eurheartj/ehz393. 10.1093/eurheartj/ehz393 PubMed 31222221
