pSBtet rtTA G72V SE Δsplice GP Luc
(Plasmid
#194328)
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PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
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Depositing Lab
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Depositing OrganizationUniversity of Otago
Located in New Zealand -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194328 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 194328-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 194328-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSBtet
- Backbone size w/o insert (bp) 6368
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Vector typeMammalian Expression, Luciferase
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Selectable markersNeomycin (select with G418) ; EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuciferase
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SpeciesSynthetic
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Insert Size (bp)1653
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MutationTet-responsive promotor related mutations: G72V in rtTA, Δsplice sites, sensitivity enhancing mutations
- Promoter Tight tet-responsive promotor
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 194328-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 194328-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBtet rtTA G72V SE Δsplice GP Luc was a gift from Alexander McLellan (Addgene plasmid # 194328 ; http://n2t.net/addgene:194328 ; RRID:Addgene_194328) -
For your References section:
Noise-Reduction and Sensitivity-Enhancement of a Sleeping Beauty-Based Tet-On System. Saunderson SC, Hosseini-Rad SMA, McLellan AD. Genes (Basel). 2022 Sep 20;13(10). pii: genes13101679. doi: 10.3390/genes13101679. 10.3390/genes13101679 PubMed 36292564
