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pAP27
(Plasmid #194706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194706 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 194706-D50 50 μg of DNA in Tris buffer $495
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DNA 194706-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2600
  • Total vector size (bp) 3500
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Linker::3xFlag::mNeonGreen::HAtag::Linker
  • Species
    Synthetic
  • Insert Size (bp)
    852

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tgctgcaaggcgattaagttgg
  • 3′ sequencing primer ctttatgcttccggctcgtatg
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAP27 was a gift from Alexandre Paix (Addgene plasmid # 194706 ; http://n2t.net/addgene:194706 ; RRID:Addgene_194706)

  • For your References section:

    Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach. Seleit A, Aulehla A, Paix A. Elife. 2021 Dec 6;10. pii: 75050. doi: 10.7554/eLife.75050. 10.7554/eLife.75050 PubMed 34870593
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