PHP.AX
(Plasmid #195218)

Purpose

AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)
Depositing Lab

Viviana Gradinaru
Publication

Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.1038/s41587-022-01648-w. ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	195218	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pUC57-mini
Vector type
Mammalian Expression, AAV

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
NEB Stable
Copy number
Unknown

Gene/Insert

Gene/Insert name
Synthetic construct isolate AAV-PHP.AX VP1 gene
Alt name
AAV-PHP.AX cap
Species
Synthetic
Promoter p41

Cloning Information

Cloning method Gibson Cloning
5′ sequencing primer unknown

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- pUC57mini-iCap-PHP.AX.gbk

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

PHP.AX was a gift from Viviana Gradinaru (Addgene plasmid # 195218 ; http://n2t.net/addgene:195218 ; RRID:Addgene_195218)
For your References section:

Spatial transcriptomics for profiling the tropism of viral vectors in tissues. Jang MJ, Coughlin GM, Jackson CR, Chen X, Chuapoco MR, Vendemiatti JL, Wang AZ, Gradinaru V. Nat Biotechnol. 2023 Jan 26. doi: 10.1038/s41587-022-01648-w. 10.1038/s41587-022-01648-w PubMed 36702899

PHP.AX (Plasmid #195218)