dpYPQ230-D156R-ABE5
(Plasmid
#195371)
-
PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 195371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepYPQ167
-
Vector typePlant Expression, CRISPR ; Gateway compatible ecTadA8e-(GGGGS)x8-dLbCas12a-D156R entry clone
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameecTadA8e-(GGGGS)x8-dLbCas12a-D156R
-
Tag
/ Fusion Protein
- 5' and 3' NLS
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
dpYPQ230-D156R-ABE5 was a gift from Yiping Qi (Addgene plasmid # 195371 ; http://n2t.net/addgene:195371 ; RRID:Addgene_195371) -
For your References section:
CRISPR-Cas12a base editors confer efficient multiplexed genome editing in rice. Cheng Y, Zhang Y, Li G, Fang H, Sretenovic S, Fan A, Li J, Xu J, Que Q, Qi Y. Plant Commun. 2023 Apr 13:100601. doi: 10.1016/j.xplc.2023.100601. 10.1016/j.xplc.2023.100601 PubMed 37060177