pInducer 21-3xFlag-hMEFV-S580X
(Plasmid #195474)

• Purpose: pInducer21 plasmid containing the human MEFV gene with the S580X mutation (stop codon leading to the truncation of the B30.2 domain of the pyrin protein) with a 3xFlag tag at the N-terminus
• Depositing Lab: Thomas Henry
• Publication: Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. doi: 10.1038/s41419-023-05745-9.

Backbone

• Vector backbone: pInducer21
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MEFV
• Alt name: MEditerranean FeVer gene
• Species: H. sapiens (human)
• Insert Size (bp): 2439
• Mutation: changed S580 to a stop codon
• Entrez Gene: MEFV (a.k.a. FMF, MEF, PAAND, TRIM20)
• Tag / Fusion Protein:
  • 3xFlag

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: gtccagtgtggtgga
• 3′ sequencing primer: GCCACTGTGCTGGATATCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry