pWPI-IRES-eGFP-Mefv iso1
(Plasmid
#92104)
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PurposeExpression in eukaryotic cells and generating stable cell lines
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepWPI-IRES-eGFP
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMefv isoform1
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SpeciesM. musculus (mouse)
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Entrez GeneMefv (a.k.a. FMF, TRIM20, pyrin)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (unknown if destroyed)
- 3′ cloning site AscI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWPI-IRES-eGFP-Mefv iso1 was a gift from Feng Shao (Addgene plasmid # 92104 ; http://n2t.net/addgene:92104 ; RRID:Addgene_92104) -
For your References section:
Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation. Gao W, Yang J, Liu W, Wang Y, Shao F. Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 10.1073/pnas.1601700113. Epub 2016 Aug 1. 10.1073/pnas.1601700113 PubMed 27482109
