hU6-pegRNA-EF-1α-Cre Template
(Plasmid
#196036)
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Purpose(Empty Backbone) The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneLenti-gibson backbone vector
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Backbone manufacturerJacks Lab
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Vector typeMammalian Expression
- Promoter hU6 and EF-1-alpha
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsStandard cloning
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hU6-pegRNA-EF-1α-Cre Template was a gift from Tyler Jacks (Addgene plasmid # 196036 ; http://n2t.net/addgene:196036 ; RRID:Addgene_196036) -
For your References section:
A prime editor mouse to model a broad spectrum of somatic mutations in vivo. Ely ZA, Mathey-Andrews N, Naranjo S, Gould SI, Mercer KL, Newby GA, Cabana CM, Rideout WM 3rd, Jaramillo GC, Khirallah JM, Holland K, Randolph PB, Freed-Pastor WA, Davis JR, Kulstad Z, Westcott PMK, Lin L, Anzalone AV, Horton BL, Pattada NB, Shanahan SL, Ye Z, Spranger S, Xu Q, Sanchez-Rivera FJ, Liu DR, Jacks T. Nat Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y. 10.1038/s41587-023-01783-y PubMed 37169967
