A prime editor mouse to model a broad spectrum of somatic mutations in vivo.
Ely ZA, Mathey-Andrews N, Naranjo S, Gould SI, Mercer KL, Newby GA, Cabana CM, Rideout WM 3rd, Jaramillo GC, Khirallah JM, Holland K, Randolph PB, Freed-Pastor WA, Davis JR, Kulstad Z, Westcott PMK, Lin L, Anzalone AV, Horton BL, Pattada NB, Shanahan SL, Ye Z, Spranger S, Xu Q, Sanchez-Rivera FJ, Liu DR, Jacks T
Nat Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y. PubMed Article
Ely ZA, Mathey-Andrews N, Naranjo S, Gould SI, Mercer KL, Newby GA, Cabana CM, Rideout WM 3rd, Jaramillo GC, Khirallah JM, Holland K, Randolph PB, Freed-Pastor WA, Davis JR, Kulstad Z, Westcott PMK, Lin L, Anzalone AV, Horton BL, Pattada NB, Shanahan SL, Ye Z, Spranger S, Xu Q, Sanchez-Rivera FJ, Liu DR, Jacks T
Nat Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196036 | hU6-pegRNA-EF-1α-Cre Template | The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre. |
| 196037 | hU6-pegRNA-EFS-mScarlet Template | The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet. |
| 196038 | Rosa26-LSL-PE2 Targeting Vector | The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA. |