pIF4
(Plasmid
#196069)
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PurposeExpresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196069 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAMBIA1300
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Vector typeCRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-Mxi1
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gRNA/shRNA sequenceempty
- Promoter MoRP27 promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIF4 was a gift from Kabin Xie (Addgene plasmid # 196069 ; http://n2t.net/addgene:196069 ; RRID:Addgene_196069) -
For your References section:
CRISPR/dCas9-Mediated Gene Silencing in Two Plant Fungal Pathogens. Zhang YM, Zheng L, Xie K. mSphere. 2023 Feb 21;8(1):e0059422. doi: 10.1128/msphere.00594-22. Epub 2023 Jan 19. 10.1128/msphere.00594-22 PubMed 36655998
