Rosa26_gRNA
(Plasmid #196138)

• Purpose: gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2
• Depositing Lab: Jason Sheltzer
• Publication: Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.

Backbone

• Vector backbone: Lenti-Cas9-gRNA-TagBFP2
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: TagBFP2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rosa26 gRNA
• gRNA/shRNA sequence: ACAGCAAGTTGTCTAACCCG
• Species: H. sapiens (human)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • Rosa26_gRNA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry