Oncogene-like addiction to aneuploidy in human cancers.
Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R, Sheltzer JM
Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521. PubMed Article
Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R, Sheltzer JM
Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195125 | Chr1q_Centromere-Targeting_gRNA | gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP |
| 195126 | Chr1q_Cassette-Integration_gRNA | gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration |
| 195127 | Chr1q_Cassette-Proximal_gRNA | gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls |
| 195128 | Chr8q_Centromere-Targeting_gRNA | gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP |
| 195129 | Chr7_Centromere-Targeting_gRNA | dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP |
| 195130 | TP53-KO_gRNA_1 | TP53-targeting gRNA in the LRG2.1 backbone |
| 195131 | TP53-KO_gRNA_2 | TP53-targeting gRNA in the LRG2.1 backbone |
| 195132 | MDM4_CRISPRi_gRNA_1 | gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry |
| 195133 | MDM4_CRISPRi_gRNA_2 | gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry |
| 195134 | MDM4_Deletion_Upstream_gRNA_1 | gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion |
| 195135 | MDM4_Deletion_Upstream_gRNA_2 | gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion |
| 195136 | MDM4_Deletion_Downstream_gRNA_1 | gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion |
| 195137 | MDM4_Deletion_Downstream_gRNA_2 | gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion |
| 195138 | EF1a_Puro_Telo_v1 | ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction |
| 195139 | EF1a_Puro_Telo_v2 | ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction |
| 195140 | pLVX-TetOne-Puro-MDM4 | Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette |
| 195141 | pLV-Bsd-CMV-hUCK | UCK2 overexpression vector with blasticidin resistance |
| 195143 | pBABE_HRASV12_Hyg | HRas G12V overexpression vector with hygroymycin resistance |
| 195194 | OR2M4_Deletion_gRNA | dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP |
| 195195 | OR6K6_Deletion_gRNA1 | dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP |
| 195196 | OR6K6_Deletion_gRNA2 | dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP |
| 195197 | OR6K6_Deletion_gRNA3 | dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP |
| 196138 | Rosa26_gRNA | gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2 |
| 196139 | AAVS1_gRNA | gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP |