pTPT
(Plasmid #1962)

• Depositing Lab: Robert Kingston
• Publication: Sif et al Genes Dev 1998 Sep 15;12(18):2842-51.

Backbone

• Vector backbone: pUC19M
• Backbone size w/o insert (bp): 2700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TPT
• Species: unknown
• Insert Size (bp): 150
• Mutation: 2 copies of 20bp artificial nucleosome positioning sequence upstream of a TATA box

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13 reverse?

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ds oligo containing 2 Shrader and Crothers GT phasing sequences at 5' end and a consensus TATA from AdMLP (about 45bp from 3' end) cloned into Mlu/Eco cut pUC19M. See sequence of insert under "Author's Map." Entered by Anthony Imbalzano. (kingston #868)