pAV-U6+27-Tornado-RhoBAST-MS2
(Plasmid
#196342)
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PurposeOverexpression of circular RhoBAST-MS2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTornado-RhoBAST-MS2
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SpeciesSynthetic
- Promoter U6+27
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned using pAV-U6+27-Tornado-Broccoli(#124360) . Please visit https://www.biorxiv.org/content/10.1101/2022.10.24.513449v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV-U6+27-Tornado-RhoBAST-MS2 was a gift from Murat Sunbul (Addgene plasmid # 196342 ; http://n2t.net/addgene:196342 ; RRID:Addgene_196342) -
For your References section:
Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging. Englert D, Burger EM, Grun F, Verma MS, Lackner J, Lampe M, Buhler B, Schokolowski J, Nienhaus GU, Jaschke A, Sunbul M. Nat Commun. 2023 Jun 30;14(1):3879. doi: 10.1038/s41467-023-39611-1. 10.1038/s41467-023-39611-1 PubMed 37391423
