pBAD_TypeIC_Cascade_cas3_invivo
(Plasmid #196400)

• Purpose: expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay
• Depositing Lab: David Taylor
• Publication: O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub 2023 Feb 16.

Backbone

• Vector backbone: pBAD
• Total vector size (bp): 9538
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cas5c
• Species: Desulfovibrio vulgaris str. Hildenborough
• Insert Size (bp): 680
• GenBank ID: 2781521 YP_009170
• Promoter: araBAD

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ctctctactgtttctccat

Gene/Insert 2

• Gene/Insert name: Cas7c
• Species: Desulfovibrio vulgaris str. Hildenborough
• Insert Size (bp): 873
• GenBank ID: 2781595 YP_009172.1
• Promoter: araBAD

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: agaacaaggagaactagcc

Gene/Insert 3

• Gene/Insert name: Cas8c
• Species: Desulfovibrio vulgaris str. Hildenborough
• Insert Size (bp): 1839
• GenBank ID: 2781582 YP_009171.1
• Promoter: araBAD

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cgtcaccggaggtgagggc

Gene/Insert 4

• Gene/Insert name: Cas3c
• Species: Desulfovibrio vulgaris str. Hildenborough
• Insert Size (bp): 2106
• GenBank ID: GenBank: AAS94335.1
• Promoter: araBAD
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: agagatgctgtaggttcga

Resource Information

• Supplemental Documents:
  • pBAD_CascadeIC_cas3.gb
• A portion of this plasmid was derived from a plasmid made by: Cas8c/Cas5c/Cas7c were cloned from addgene plasmid #81185

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry