pCW57.1 Cas9 P2A CRTC3-GFP
(Plasmid
#196674)
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PurposeDox controlled, conditional expression of Cas9 and a GFP-CRTC3 fusion protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCW57.1
- Backbone size w/o insert (bp) 7300
- Total vector size (bp) 14314
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas9 P2A hCRTC3-EGFP
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SpeciesH. sapiens (human)
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Entrez GeneCRTC3 (a.k.a. TORC-3, TORC3)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW57.1 Cas9 P2A CRTC3-GFP was a gift from David E. Fisher (Addgene plasmid # 196674 ; http://n2t.net/addgene:196674 ; RRID:Addgene_196674)