PurposeRep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||196683||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 9398
Modifications to backboneBackbone modifications as in kiCAP-AAV-BI30
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
Insert Size (bp)2232
MutationPTQGTVR insert between amino acids 588 and 589 of AAV9 VP1
- Promoter p41
- Cloning method Restriction Enzyme
- 5′ cloning site BsiWI (not destroyed)
- 3′ cloning site AgeI (destroyed during cloning)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:kiCAP-AAV-PAL1A was a gift from Pardis Sabeti (Addgene plasmid # 196683 ; http://n2t.net/addgene:196683 ; RRID:Addgene_196683)
For your References section:Systemic administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque CNS. Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, Solomon IH, Tabebordbar M, Sabeti PC. Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002. 10.1016/j.medj.2022.11.002 PubMed 36417917