kiCAP-AAV-PAL1B
(Plasmid #196684)

• Purpose: Rep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.
• Depositing Lab: Pardis Sabeti
• Publication: Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002.

Backbone

• Vector backbone: pUC57-Kan
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 9398
• Modifications to backbone: Backbone modifications as in kiCAP-AAV-BI30
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
• Alt name: PAL1B
• Species: Synthetic
• Insert Size (bp): 2232
• Mutation: PSQGTLR insert between amino acids 588 and 589 of AAV9 VP1
• Promoter: p41

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsiWI (not destroyed)
• 3′ cloning site: AgeI (destroyed during cloning)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • kiCAP-AAV-PAL1B.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry