pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover
(Plasmid
#196829)
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PurposeExpress dpdpCas13b with AAAA mutation and Clover
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196829 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAC90-pmaxGW
- Backbone size w/o insert (bp) 2955
- Total vector size (bp) 6431
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedpspCas13b(AAAA)
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Insert Size (bp)3464
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MutationK367A, D369A and K370A
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Tags
/ Fusion Proteins
- 3XFLAG (N terminal on insert)
- NES (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.497089v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover was a gift from Albert Cheng (Addgene plasmid # 196829 ; http://n2t.net/addgene:196829 ; RRID:Addgene_196829) -
For your References section:
Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold. Liu Z, Jillette N, Robson P, Cheng AW. Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547. 10.1093/nar/gkad547 PubMed 37395412
Map uploaded by the depositor.
