Skip to main content
Addgene
Open Menu

pEGFP-survivin
(Plasmid #196908)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196908 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 196908-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 196908-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5100
  • Modifications to backbone
    EcoR I and Sal I sites of pEGFP-ΔC2, which had been derived from pEGFP-C1 by cutting and filling-in Hind III site and converted to C2 reading frame
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    survivin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Mutation
    E129K
  • GenBank ID
    NM_001168
  • Entrez Gene
    BIRC5 (a.k.a. API4, EPR-1)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    OriGene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

400bp BamHI-EcoRI fragment of pcDNA3-survivin was inserted into Bgl II and EcoRI sites of EGFP-C1

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-survivin was a gift from Kenji Sugimoto (Addgene plasmid # 196908 ; http://n2t.net/addgene:196908 ; RRID:Addgene_196908)

  • For your References section:

    Visualization of chromosomes and nuclear envelope in living cells for molecular dynamics studies. Fukada T, Inoue K, Urano T, Sugimoto K. Biotechniques. 2004 Oct;37(4):552, 554, 556. doi: 10.2144/04374BM05. 10.2144/04374BM05 PubMed 15517965
Related items:
Commonly requested with: