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pgLAP1
(Plasmid #19702)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19702 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 7624
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • TEV (N terminal on backbone)
    • S peptide (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (not destroyed)
  • 3′ cloning site Gateway (not destroyed)
  • 5′ sequencing primer ATC ACT CTC GGC ATG GAC GAG CTG TAC AAG
  • 3′ sequencing primer TGG CTG GCA ACT AGA AGG CAC AGT CGA GGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgLAP1 was a gift from Peter Jackson (Addgene plasmid # 19702 ; http://n2t.net/addgene:19702 ; RRID:Addgene_19702)