pMAT_CD28ICD
(Plasmid
#197098)
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PurposeThis plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMAT
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Backbone manufacturerThermofisher
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameThis plasmid contains the coding sequence for the intracellular domain of CD28.
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byCD8a hinge and transmembrane domain: Provided by Michael Milone and Dr. Carl June UPENN.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAT_CD28ICD was a gift from Wendell Lim (Addgene plasmid # 197098 ; http://n2t.net/addgene:197098 ; RRID:Addgene_197098) -
For your References section:
Decoding CAR T cell phenotype using combinatorial signaling motif libraries and machine learning. Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W, Bianco S, Lim WA. Science. 2022 Dec 16;378(6625):1194-1200. doi: 10.1126/science.abq0225. Epub 2022 Dec 8. 10.1126/science.abq0225 PubMed 36480602
