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(Plasmid #197102)


Item Catalog # Description Quantity Price (USD)
Plasmid 197102 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    MoBiTec GmbH, Germany
  • Total vector size (bp) 3800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    TyrRS variant, amber suppressor tRNA
  • Species
    Methanocaldococcus jannaschii (TyrRS, tRNA)
  • Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (TyrRS with promoter), SphI(tRNA with promoter) (unknown if destroyed)
  • 3′ cloning site BglII (TyrRS), SalI (tRNA with terminator) (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBpaRS3tRNA was a gift from Kensaku Sakamoto (Addgene plasmid # 197102 ; ; RRID:Addgene_197102)
  • For your References section:

    Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid. Sato S, Mimasu S, Sato A, Hino N, Sakamoto K, Umehara T, Yokoyama S. Biochemistry. 2011 Jan 18;50(2):250-7. doi: 10.1021/bi1016183. Epub 2010 Dec 16. 10.1021/bi1016183 PubMed 21128684