PurposeExpresses the fusion protein of CytERM-mNeptune2.5 in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||197194||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesM. musculus (mouse)
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.researchsquare.com/article/rs-797586/v1 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCytERM-mNeptune2.5-N1 was a gift from Kiryl Piatkevich (Addgene plasmid # 197194 ; http://n2t.net/addgene:197194 ; RRID:Addgene_197194)
For your References section:Quantitative assessment of near-infrared fluorescent proteins. Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, Koster RW, Wu L, Namikawa K, Piatkevich KD. Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z. 10.1038/s41592-023-01975-z PubMed 37666982