pBBR1.cas9-NT
(Plasmid #197871)

• Purpose: Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
• Depositing Lab: Baojun Wang
• Publication: Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-721. doi: 10.1021/acssynbio.2c00465. Epub 2023 Feb 20.

Backbone

• Vector backbone: Bba_J72114
• Backbone manufacturer: Christopher Anderson
• Backbone size w/o insert (bp): 9334
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: trancCRNA
• Species: Streptococcus pyogenes
• Insert Size (bp): 214

Gene/Insert 2

• Gene/Insert name: Cas9
• Alt name: SpCas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 4107

Gene/Insert 3

• Gene/Insert name: crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
• Species: Streptococcus pyogenes
• Insert Size (bp): 255

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry