piggybac-EF1-halo-Oct4
(Plasmid #197909)

• Purpose: single-particle tracking for OCT4
• Depositing Lab: James Zhe Liu
• Publication: Dong et al bioRxiv 2022.10.26.513917

Backbone

• Vector backbone: pBR322
• Backbone manufacturer: synthetic
• Backbone size w/o insert (bp): 7872
• Total vector size (bp): 9010
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pou5f1
• Alt name: pou5f1, oct4
• Species: M. musculus (mouse)
• Insert Size (bp): 1062
• GenBank ID: NM_001252452.1 NP_038661.2
• Entrez Gene: Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
• Promoter: EF1
• Tag / Fusion Protein:
  • Halotag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: tttgcctgaccctgcttgc

Resource Information

• Supplemental Documents:
  • piggybac-EF1-halo-Oct4.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.10.26.513917v1 for bioRxiv preprint.

Addgene QC found a deletion in EF1a promoter that will not affect plasmid function.