|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19842||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerS Rees et al., BioTechniques 1996
- Backbone size w/o insert (bp) 5257
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMKL1 S449A
Alt nameMAL, BSAC, MRTF-A
SpeciesH. sapiens (human)
Insert Size (bp)2800
Mutationchanged Serine 449 to Alanine
Entrez GeneMRTFA (a.k.a. BSAC, MAL, MKL, MKL1, MRTF-A)
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCin4-3xFLAG-MKL1 S449A was a gift from Ron Prywes (Addgene plasmid # 19842 ; http://n2t.net/addgene:19842 ; RRID:Addgene_19842)
For your References section:Serum-induced phosphorylation of the SRF coactivator MKL1 by the ERK1/2 pathway inhibits its nuclear localization. Muehlich S, Wang R, Lee SM, Lewis TC, Dai C, Prywes R. Mol Cell Biol. 2008 Aug 11. ():. 10.1128/MCB.00427-08 PubMed 18694962
Map uploaded by the depositor.