vHB63
(Plasmid
#198748)
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Purpose(Empty Backbone) pneo650-NAT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 198748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonevc241
- Backbone size (bp) 6300
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Vector typeKnockout pandoravirus/mollivirus
- Promoter pneo650
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Selectable markersnourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
vHB63 was a gift from Chantal Abergel (Addgene plasmid # 198748 ; http://n2t.net/addgene:198748 ; RRID:Addgene_198748) -
For your References section:
Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii. Philippe N, Shukla A, Abergel C, Bisio H. Nat Protoc. 2023 Nov 14. doi: 10.1038/s41596-023-00910-y. 10.1038/s41596-023-00910-y PubMed 37964008
