pCA11-pMa-sgTS3-17xMS2V5-sgTS3
(Plasmid
#199442)
-
PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepUC19
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHITI donor template
-
SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCA11-pMa-sgTS3-17xMS2V5-sgTS3 was a gift from Baohui Chen (Addgene plasmid # 199442 ; http://n2t.net/addgene:199442 ; RRID:Addgene_199442) -
For your References section:
Real-time imaging of RNA polymerase I activity in living human cells. Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B. J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 10.1083/jcb.202202110. Epub 2022 Oct 25. 10.1083/jcb.202202110 PubMed 36282216
