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Real-time imaging of RNA polymerase I activity in living human cells.
Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B
J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 10.1083/jcb.202202110. Epub 2022 Oct 25. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199442pCA11-pMa-sgTS3-17xMS2V5-sgTS3CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms
199443pCA12-pHR-SFFV-BFP-B23Expression of BFP-B23 in mammalian cells
199444pCA13-pHR-SFFV-HaloTag-FBLExpression of HaloTag-FBL in mammalian cells
199445pCA14-pHR-SFFV-GFP-RPA43Expression of GFP-RPA43 in mammalian cells

Antibodies from Article