GFP SMG1 T3550A/S3556A
(Plasmid #199572)

• Purpose: Expresses mutated SMG1 fused to GFP
• Depositing Lab: Michael Kastan
• Publication: McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 15700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMG1
• Species: H. sapiens (human)
• Insert Size (bp): 11000
• Mutation: T3550A and S3556A
• GenBank ID: NM_015092
• Entrez Gene: SMG1 (a.k.a. 61E3.4, ATX, LIP)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry