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Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced Alternative Splicing Pathway.
McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB
Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199571GFP SMG1 WTExpresses WT SMG1 fused to GFP
199572GFP SMG1 T3550A/S3556AExpresses mutated SMG1 fused to GFP
199573FLAG SMG1 WTExpresses FLAG-tagged WT SMG1
199574FLAG SMG1 T3550A/S3556AExpresses FLAG-tagged mutated SMG1
199575FLAG SMG1 D2335A/N2340KExpresses FLAG-tagged kinase dead SMG1
199576DDX5 WT V5Expresses WT DDX5 cloned into pLenti puro
199577DDX5 D248N V5Expresses helicase dead DDX5 cloned into pLenti puro

Antibodies from Article