pmKO-imp α
(Plasmid #200082)

• Purpose: Expresses mKO-tagged human importinα in mammalian cells
• Depositing Lab: Kenji Sugimoto
• Publication: Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7.

Backbone

• Vector backbone: pmKO1-MC1
• Backbone manufacturer: MBL
• Backbone size w/o insert (bp): 4600
• Total vector size (bp): 5700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: importin α
• Alt name: KPNA2
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Promoter: CMV
• Tag / Fusion Protein:
  • mKO (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: mKO C-terminal side
• 3′ sequencing primer: SV40 poly A side

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The DNA fragment of imp α is the same as the one cloned in pEGFP-imp α
Plasmid vector pmKO1-MC1 is the same as the pmKO-AF8 except for the cloning sites.