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pBI-eGFP-Rab22
(Plasmid #200264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200264 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 200264-D50 50 μg of DNA in Tris buffer $495
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DNA 200264-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBI
  • Backbone manufacturer
    Clontech/Takara Bio
  • Total vector size (bp) 4358
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab22a (human cDNA)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    585
  • Entrez Gene
    RAB22A
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer atggcgctgagggagctc
  • 3′ sequencing primer CGGCCGCGTCGACCTAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rab22a cDNA was from UMR cDNA resource center

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI-eGFP-Rab22 was a gift from Guangpu Li (Addgene plasmid # 200264 ; http://n2t.net/addgene:200264 ; RRID:Addgene_200264)

  • For your References section:

    A novel membrane targeting domain mediates the endosomal or Golgi localization specificity of small GTPases Rab22 and Rab31. Banworth MJ, Liang Z, Li G. J Biol Chem. 2022 Sep;298(9):102281. doi: 10.1016/j.jbc.2022.102281. Epub 2022 Jul 19. 10.1016/j.jbc.2022.102281 PubMed 35863437
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