pBI-eGFP-Rab31
(Plasmid #200265)

• Purpose: Expression of eGFP-Rab31 (human) in mammalian cells
• Depositing Lab: Guangpu Li
• Publication: Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi: 10.1016/j.jbc.2022.102281. Epub 2022 Jul 19.

Backbone

• Vector backbone: pBI
• Backbone manufacturer: Clontech/Takara Bio
• Backbone size w/o insert (bp): 4358
• Total vector size (bp): 5663
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab31 (human cDNA)
• Species: H. sapiens (human)
• Insert Size (bp): 588
• Entrez Gene: RAB31 (a.k.a. Rab22B)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: atgatggcgatacgggag
• 3′ sequencing primer: tcaacagcaccggcg

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Rab31 cDNA was from UMR cDNA resource center

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry