CMV mScarlet-LC3B
(Plasmid
#200431)
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PurposeMammalian expression of fluorescently-tagged marker for autophagic vesicles
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Depositing Lab
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Depositing OrganizationUniversity of Pennsylvania
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 200431-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 200431-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFPc
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3900
- Total vector size (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMap1lc3b
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Alt namemicrotubule associated protein 1 light chain 3 beta
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Alt nameLC3B
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Alt nameMpl3
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1500
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GenBank IDNC_051354.1
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Entrez GeneMap1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer 5'-CTTTATTTGTAACCATTATAAGCTGC
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEGFP-LC3B; gift from T. Yoshimori, Osaka University
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 200431-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 200431-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV mScarlet-LC3B was a gift from Erika Holzbaur (Addgene plasmid # 200431 ; http://n2t.net/addgene:200431 ; RRID:Addgene_200431) -
For your References section:
Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and ARF6 GTPase disrupts the axonal transport of autophagosomes. Dou D, Smith EM, Evans CS, Boecker CA, Holzbaur ELF. Cell Rep. 2023 May 1;42(5):112448. doi: 10.1016/j.celrep.2023.112448. 10.1016/j.celrep.2023.112448 PubMed 37133994
Map uploaded by the depositor.
