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(Plasmid #200779)


Item Catalog # Description Quantity Price (USD)
Plasmid 200779 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6293
  • Total vector size (bp) 7796
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
  • Promoter Ptwk-40
  • Tag / Fusion Protein
    • mNeptune (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tggctagcgtcgacggatccaaaaaatgggatctcaccaccaccaccaccacggaa
  • 3′ sequencing primer ttccgtggtggtggtggtggtgagatcccattttttggatccgtcgacgctagcca
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

Please note: This plasmid contains a 62 bp deletion in unc-54 3' UTR. This deletion is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH4542 was a gift from Mei Zhen (Addgene plasmid # 200779 ; ; RRID:Addgene_200779)
  • For your References section:

    A tonically active master neuron modulates mutually exclusive motor states at two timescales. Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen Q, Boulin T, Gao S, Zhen M. Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. Epub 2024 Apr 10. 10.1126/sciadv.adk0002 PubMed 38598630