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Depositing Lab
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Depositing OrganizationNational Institute of Allergy and Infectious Diseases (NIAID)
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20126 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 20126-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 20126-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL4.10
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4242
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemIL-17
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Alt namemIL-17 promoter
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Alt nameIL17
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SpeciesM. musculus (mouse)
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Insert Size (bp)600
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GenBank IDAC159614
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Entrez GeneIl17a (a.k.a. Ctla-8, Ctla8, IL-17, IL-17A, Il17)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 20126-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 20126-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4 mIL-17 0.6kb promoter was a gift from Warren Strober (Addgene plasmid # 20126 ; http://n2t.net/addgene:20126 ; RRID:Addgene_20126) -
For your References section:
Interactions between the transcription factors Runx1, RORgt and Foxp3 regulate differentiation of interleukin 17-producing T cells. Fuping Zhang, Guangxun Meng & Warren Strober. 2008 Nov;9(11):1297-306. Epub 2008 Oct 12. 10.1038/ni.1663 PubMed 18849990