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Addgene

pAAV-U6-AlbEx14-mNG
(Plasmid #201780)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201780 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV.GFAP.Cre.WPRE.hGH
  • Backbone manufacturer
    Addgene plasmid #105550
  • Backbone size w/o insert (bp) 2876
  • Total vector size (bp) 7454
  • Vector type
    AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Alb gRNA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3226
  • Entrez Gene
    Alb (a.k.a. Alb-1, Alb1, BCL002)
  • Promoter U6

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Alb_LHA-mNeonGreen-Alb_RHA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Alb (a.k.a. Alb-1, Alb1, BCL002)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-U6-AlbEx14-mNG was a gift from Hajime Hirase (Addgene plasmid # 201780 ; http://n2t.net/addgene:201780 ; RRID:Addgene_201780)
  • For your References section:

    Virally induced CRISPR/Cas9-based knock-in of fluorescent albumin allows long-term visualization of cerebral circulation in infant and adult mice. Vittani M, Knak PAG, Fukuda M, Nagao M, Wang X, Kjaerby C, Konno A, Hirai H, Nedergaard M, Hirase H. Fluorescence Imaging of the Brain. Neuromethods, vol 209. Humana, New York, NY. 10.1007/978-1-0716-4011-1_6