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h3.7SP-Crtta
(Plasmid #20180)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20180 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 20180-D50 50 μg of DNA in Tris buffer $495
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DNA 20180-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    h3.7SP-Crtta
  • Alt name
    Sftpc-reverse tetracycline transactivator
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5100
  • Entrez Gene
    SFTPC (a.k.a. BRICD6, PSP-C, SFTP2, SMDP2, SP-C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13_forward20
  • 3′ sequencing primer M13rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We cloned the promoter (3.7SP-C), Tet Systems the rtTA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used to express genes in the lung epithelium or lung epithelial cells

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    h3.7SP-Crtta was a gift from Jeffrey Whitsett (Addgene plasmid # 20180 ; http://n2t.net/addgene:20180 ; RRID:Addgene_20180)

  • For your References section:

    Conditional expression of fibroblast growth factor-7 in the developing and mature lung. Tichelaar JW, Lu W, Whitsett JA. J Biol Chem. 2000 Apr 21. 275(16):11858-64. 10.1074/jbc.275.16.11858 PubMed 10766812
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