PurposeLentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51293||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLuigi Naldini
- Backbone size w/o insert (bp) 6697
Growth in Bacteria
Copy numberHigh Copy
Alt nameHistone 2B-enhanced yellow fluorescent protein
SpeciesH. sapiens (human)
Insert Size (bp)1116
Entrez GeneH2BC21 (a.k.a. GL105, H2B, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
- Promoter mTie1
/ Fusion Protein
- H2B-eYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site BsrGI (destroyed during cloning)
- 5′ sequencing primer not known
- 3′ sequencing primer not known (Common Sequencing Primers)
Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti.tie1.H2B.eYFP.W was a gift from Rusty Lansford (Addgene plasmid # 51293 ; http://n2t.net/addgene:51293 ; RRID:Addgene_51293)
For your References section:Dynamic analysis of vascular morphogenesis using transgenic quail embryos. Sato Y, Poynter G, Huss D, Filla MB, Czirok A, Rongish BJ, Little CD, Fraser SE, Lansford R. PLoS One. 2010 Sep 14;5(9):e12674. doi: 10.1371/journal.pone.0012674. 10.1371/journal.pone.0012674 PubMed 20856866