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(Plasmid #51293)


Item Catalog # Description Quantity Price (USD)
Plasmid 51293 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Luigi Naldini
  • Backbone size w/o insert (bp) 6697
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Histone 2B-enhanced yellow fluorescent protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter mTie1
  • Tag / Fusion Protein
    • H2B-eYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site BsrGI (destroyed during cloning)
  • 5′ sequencing primer not known
  • 3′ sequencing primer not known
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Original plasmid was a kind gift from Dr. Luigi Naldini
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti.tie1.H2B.eYFP.W was a gift from Rusty Lansford (Addgene plasmid # 51293 ; ; RRID:Addgene_51293)
  • For your References section:

    Dynamic analysis of vascular morphogenesis using transgenic quail embryos. Sato Y, Poynter G, Huss D, Filla MB, Czirok A, Rongish BJ, Little CD, Fraser SE, Lansford R. PLoS One. 2010 Sep 14;5(9):e12674. doi: 10.1371/journal.pone.0012674. 10.1371/journal.pone.0012674 PubMed 20856866