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Addgene

Anti-c-Myc [9E10] - Chimeric
(Antibody #201857)

  • Purpose
    Anti-c-Myc chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rat IgG2a Fc.
  • Depositing Lab
  • Publication

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 201857-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 201857-rAb.T Limited Stock Available, 4 units left
20 µg of purified recombinant antibody
$85 *

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Features

Production & Usage

  • Antigen Plasmid
    pAAV.CAG.GFPsm-myc.WPRE.SV40
  • Purification
    Purified from cell culture supernatant using affinity chromatography followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2938598

Terms and Licenses

Target Antigen

Protein Myc proto-oncogene protein
Antigen Description Amino acids 408–432 of human Myc
Antigen Amino Acid Sequence
AEEQKLISEEDLLRKRREQLKHKLE
Species H. sapiens (human)
Gene MYC
Alternative Names
  • Class E basic helix-loop-helix protein 39 (bHLHe39)
  • Proto-oncogene c-Myc
  • Transcription factor p64
External References

Applications (1)

Western Blot

1 image
Western Blot image by Addgene
Submitted By: Addgene
Antibody Type
Recombinant
Sample Species
H. sapiens (human)
Result
Pass

Addgene Comments

This antibody has been engineered from sequences from a mouse monoclonal parent antibody. By necessity, some mouse sequences are retained. When multiplexing with mouse antibodies, we recommend using Fc-directed, pre-adsorbed secondary antibodies.
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-c-Myc [9E10] - Chimeric - from Melina Fan (Addgene antibody # 201857 ; http://n2t.net/addgene:201857 ; RRID:AB_2938598)
  • For your References section: