pL0_CD-eYFP
(Plasmid #202139)

• Purpose: Level 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.
• Depositing Lab: Christopher Dupont
• Publication: Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. Epub 2020 Feb 5.

Backbone

• Vector backbone: pL0R
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eYFP gene
• Species: Aequorea victoria
• Insert Size (bp): 724
• Mutation: None

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pL0_CD-eYFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry