pBacPAK-HFT-dKDM4B
(Plasmid #20234)

• Depositing Lab: Jerry Workman
• Publication: Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Backbone

• Vector backbone: pBacPAK-HFT
• Backbone size w/o insert (bp): 5608
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dKDM4B
• Alt name: CG33182
• Species: D. melanogaster (fly)
• Insert Size (bp): 1359
• GenBank ID: NM_176164
• Entrez Gene: Kdm4B (a.k.a. Dmel_CG33182, CG33182, CG4037, Dmel\CG33182, KDM4B, dJMJD2(2), dKDM4B, dKdm4B)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • FLAG (N terminal on backbone)
  • TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ccattgtaatgagacgca
• 3′ sequencing primer: ACGCACAGAATCTAGCGCT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TEV tag ENPYFQG instead of ENLYFQG.

The cDNA used in making dKDM4B(CG33182) constructs has incomplete 3' end. It is because NCBI data has updated the sequence after I made the construct. There is a comment on NCBI page https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=161077032&from=148&to=1920&view=gbwithparts indicates that they replaced a former sequence of CG33182 https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=28573833 with the current 1773bp one.