pattB-LSL-AAVR-F2A-spCas9
(Plasmid #202459)

• Purpose: The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.
• Depositing Lab: Jan Carette
• Depositing Organization: Stanford University
  Located in the United States of America
• Publication: Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. doi: 10.1038/s41592-023-01896-x. Epub 2023 Jun 8.

Backbone

• Vector backbone: pBT378, Addgene, cat#52554
• Backbone manufacturer: Liqun Luo
• Backbone size w/o insert (bp): 5510
• Total vector size (bp): 16316
• Modifications to backbone: Previously modified by Monte Winslow to express Streptococcus pyogenes Cas9 (spCas9) with a LSL sequence proceeding to generate paatB-LSL-spCas9.
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAVR
• Alt name: AU040320
• Alt name: Kiaa0319L
• Species: M. musculus (mouse)
• Insert Size (bp): 3144
• Entrez Gene: AU040320 (a.k.a. A730047D20Rik, AAVR, Kiaa0319l)
• Promoter: CAG
• Tag / Fusion Protein:
  • mCherry fusion (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gtaaaacgacggccagt
• 3′ sequencing primer: gtcatagctgtttcctg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 202459-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 202459-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.